By Tara L. Fulton (auth.), Beth Shapiro, Michael Hofreiter (eds.)
Research into historic DNA all started greater than 25 years in the past with the booklet of brief mitochondrial DNA series fragments from the quagga, an extinct relative of the zebra. historic DNA examine quite won momentum following the discovery of PCR, which allowed thousands of copies to be made from the few closing DNA molecules preserved in fossils and museum specimens. In Ancient DNA: tools and Protocols professional researchers within the box describe a few of the protocols which are now regularly occurring to review old DNA. those comprise directions for developing an historical DNA laboratory, extraction protocols for quite a lot of assorted substrates, info of laboratory strategies together with PCR and NGS library coaching, and proposals for acceptable analytical techniques to make feel of the sequences received. Written within the hugely profitable Methods in Molecular Biology™ sequence layout, chapters comprise introductions to their respective themes, lists of the required fabrics and reagents, step by step, easily reproducible laboratory protocols, and key pointers on troubleshooting and warding off recognized pitfalls.
Authoritative and useful, Ancient DNA: tools and Protocols seeks to help scientists within the extra learn of old DNA and the methodological techniques in historical research.
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